The first-leg was a very closely contested affair with just two goals.
Thursday's return leg at the Stadio San Paolo could once again be a cagey contest.
With both teams being two of the favourites to win the competition, either side will be desperate to avoid elimination on Thursday.
Aubameyang announced his arrival at Barca by scoring a hat-trick against Valencia.
The striker will be keen to lead Xavi's side to European glory this season and is now full of confidence.
Match preview: SSC Napoli vs FC Barcelona
Napoli and Barcelona head into the second-leg of their Europa League play off match with the tie finely poised.
Both teams played out a 1-1 draw at Camp Nou last week.
Thursday's hosts took the lead through Piotr Zielinski.
However, new signing Ferran Torres responded for Barca, as he netted from the penalty spot.
Napoli are enjoying a strong domestic campaign as they sit 3rd in Serie A and just two points off the top.
Under Xavi, Barcelona are shown great signs of improvement and are now back in the top four.
Barca claimed a huge 4-1 win over Valencia at the Mestalla Stadium over the weekend.
SSC Napoli team news
Giovanni Di Lorenzo and Kevin Malcuit are both doubts for the match having picked up knocks at the weekend.
They could join Axel Tuanzebe, Stanislav Lobotka, Andre-Frank Zambo Anguissa and Hirving Lozano on the sidelines.
FC Barcelona team news
Dani Alves is ineligible for the Europa League as Barcelona opted against registering him in their squad.
However, Ronald Araujo returns from a calf injury in a big boost for the visitors.
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